12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality. CYT10 strain was identified based on the morphological and molecular characteristics. Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced. The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana (>95%). Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain, which was belonging to B. Bassiana. CYT10 strain was classified as a member of the species B. Bassiana based on the morphological characteristics and analysis of DNA sequences.%从草履蚧僵虫体内分离出12株病原真菌,对蓟马类昆虫具有70%～90%的致死率.现选出一株病原真菌CYT10利用形态和分子的特点进行鉴定.扫描电镜下观察,该菌产孢细胞簇生瓶状,“之”型产孢轴.经PCR获得rDNA-ITS和EF1 -a核苷酸序列,与其他球孢白僵菌的相应序列一致性达95%以上,进化分析CYT10菌株与球孢白僵菌Beauveria bassiana聚为一类.根据菌株的形态观察和DNA序列分析确定该菌为球孢白僵菌.
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