茶树在干旱条件下的mRNA差异表达

摘要

20% PEG-6000 was used for simulating drought treatment to Camellia sinensis cv. Fuding-dabaicha clone. mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) was used to study the mRNA differential expression between drought stress and irrigated control. One down regulated gene was found under drought stress. Sequence analysis and homology alignment showed that N3.5 showed 75% homogenicity with the Camellia sinensis clone Sajin tea leaf mutant color tag S31.B15 genomic sequence (Accession: DQ443473.1). It was suggested that the fragments N3.5 was related to drought tolerance mechanism of tea plant according to the functions of their homologous sequences.%采用20％PEG-6000对福鼎大白茶无性系扦插苗进行模拟干旱处理,应用mRNA差异显示技术研究干旱与正常浇水对照的mRNA差异表达,发现1个在干旱条件下减量表达片段N3-5.序列分析和同源性比对表明,N3-5与茶树无性系Sajin茶叶色突变基因组序列标签S31.B15 (Accession:DQ443473.1)有75％的同源性.根据序列相似同源基因的功能推测,该片段可能与茶树的抗旱机制有关.