茶黄素对 BSA/MDA 羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究

摘要

A model of age pigment generation was builted by carbonyl-ammonia cross linking reaction in vitro, the datas indicate that theaflavins can not only inhibit the generation of age pigment in protein carbonylation, but also supress β-sheet-rich in aggregate protein in this model by thioflavin T-based fluorometric assay, scanning electron microscope, transmission electric microscope, SDS-PAGE and NBT staining, there are postive relationship between protein carbonylation on mechanism of aging and protein aggregation that lead to neurodegenerative disease.%本实验构建了羰-氨交联反应形成老年色素的体外反应模型，通过 ThT 荧光、扫描电子显微镜、透射电镜、SDS-PAGE 及 NBT 染色等检测手段，结果表明，茶黄素不仅可以抑制此模型中由于蛋白质羰基化产生的老年色素，还可以抑制此模型中因蛋白质聚集化产生的β-sheet 结构。上述结果揭示了衰老机制中的蛋白质羰基化与导致神经退行性疾病的蛋白质聚集化之间存在一定的相关性。