[Objective] To determine the effect of a kind of small molecule drug (N-acetyl cysteine, NAC) on the expansion of CD4+ central memory T cells (TCM) in vitro and improve the effect of adoptive immunotherapy.[Methods] Human peripheral CD4+ T lymphocytes were isolated from health human blood and divided into two groups (control group and experimental group), meanwhile, NAC was only added into experimental group. After that, the expansion of CD4+ TCM was detected by flow cytometry and cell counter. [Results] 10 mmol/L NAC was optimal concentration for the expansion of CD4+ T cells in vitro. NAC does not have any significant effect on the ratio of CD4+ TCM to the whole CD4+ T cells; After 15 days of amplification, we counted the number of CD4+ TCM: in control group, the number was (7.24 ± 0.54)×105 compared with (5.13 ± 0.47) × 106 in experiment group, in which the absolute cell number was amplified about seven folds. Statistical analysis (t test) about the numbers of CD4+ TCM was carried out between these two groups and the difference is statistically significant (P < 0.01). Through cell apoptosis detection, we found that the proportion of apoptotic cells in experimental group is lower than that in control group. [Conclusions] NAC has significant effect on the expansion of CD4+ TCM and it provides a simple, safe and effective expansion method in vitro for adoptive immunotherapy.%[目的]探讨小分子药物(N-乙酰半胱氨酸,NAC)对CD4+中央记忆性T细胞(TCM)体外扩增的影响从而改善过继免疫治疗的效果.[方法]体外分离人类的外周血CD4+T淋巴细胞,将细胞分为两组(实验组和对照组)进行细胞培养,实验组加入N-乙酰半胱氨酸后,通过流式细胞仪和细胞计数仪对两组细胞中的CD4+ TCM的扩增情况进行检测.[结果]在CD4+T细胞体外扩增实验中,10 mmol/L的NAC为促进细胞扩增的最佳浓度；NAC对TCM在CD4+T细胞中的比例无明显的影响;在CD4+T细胞扩增的第15天检测其中TCM的细胞数量,对照组中TCM的数量为(7.24±0.54)×105,实验组中TCM的数量为(5.13±0.47)×106,较之对照组,其TCM的数量有着7倍左右的扩增；对上述两组数据进行统计学分析(t检验),P＜0.01,表明差异具有统计学意义；对上述两组细胞进行凋亡检测,发现实验组中凋亡细胞的比例低于对照组.[结论]NAC可有效地促进CD4+ TCM细胞的扩增,为过继免疫治疗提供简单、安全及有效的体外扩增方法.
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