山羊腰椎间盘损伤后P物质免疫染色阳性神经纤维的分布

摘要

Objective To study the histology changes and the expression of substance P (SP) immunopositive nerve fibers of goat intervertebral disc after lesion. Methods Fifteen goats were subjected to surgery by puncture knife ( 1.2 mm diameter), and the inner side of posterior annulus fibrosus of L6/L7 intervertebral discs was penetrated as the experimental intervertebral disc. L5/L6 intervertebral discs were penetrated at the anterior side of intervertebral disc as the positive control intervertebral disc, while L4/L5 intervertebral discs were as the intact control intervertebral disc. All goats were put to death randomly at 3 weeks, 3 months and 6 months after operation respectively (5 goats at each time). The histology changes of posterior annulus fibrosus and the expression of SP immunopositive nerve fibers were observed. Results All L6/L7 posterior internal annulus fibrosus disruption were not healed in 3 weeks after lesion. At 3 months and 6 months, SP immunopositive nerve fibers were observed in the right posterior outer 1/4 regions of L6/L7 discs easily, which were further ingrowth into the disc along the puncture tract and periphery tissue. But immunopositive nerve fiber was absent in the same of regions of L4/L5/L6 discs.Conclusion After stabbing the posterior internal annulus fibrosus through goat anterior lumbar discs, posterior internal annulus fibrosus disruption is hard to heal and SP immunopositive nerve fibers appear.%目的 研究山羊椎间盘损伤后的组织学改变及P物质(substance P,SP)免疫染色阳性神经纤维在损伤纤维中的分布.方法 15只山羊,用直径1.2 mm的穿刺针刀刺伤L5/L6椎间盘前纤维环全层和L6/L7后部纤维环内侧,以L4/L5椎间盘作为完整对照椎间盘.分别于损伤后3周、3个月、6个月随机处死5只山羊.观察实验椎间盘后部纤维环组织学改变和SP免疫染色阳性神经纤维的分布.结果 L6/L7椎间盘后部纤维环内层损伤后3周均未愈合.损伤3个月及6个月时,在L6/L7椎间盘的右后外1/4区很容易检测到SP免疫染色阳性神经纤维.神经纤维沿穿刺通道及周围组织向内生长.L4/L5/L6椎间盘相同区域未检测到相关免疫染色阳性反应神经纤维.结论 经山羊椎间盘前方损伤后部纤维环内层后,纤维环内层很难愈合,并出现SP免疫染色阳性神经纤维分布.