采用RAPD分子标记技术分析9个自然居群米心水青冈的遗传关系和多样性.20条随机引物共扩出260个条带,平均每条引物扩增出13个条带,其DNA带的分子量在200～3000 bp.其中多态性条带有180条,多态位点比率(PPL)为69.2％.种内Shannon多样性指数为0.302 1,群体内所占比例为72.10％,群体间所占比例为27.90％.种内Nei's基因多样性为0.2045,群体内平均基因多样性为0.1502,基因分化系数GST为0.2631.米心水青冈的遗传多样性水平处于中等,遗传分化也处于中等水平.%The genetic relationship and genetic diversity of nine natural populations of Fagus engleriana from four provinces were analyzed by random amplified polymorphic DNA ( RAPD) marker technique. Totally 260 bands were amplified by 20 primers, 13 bands were averagely amplified by each primer. The molecular weight of the band DNA was between 200 bp and 3 000 bp. Out of the 260 bands, 180 bands were polymorphic and the percentage of polymorphic loci (PPL) was 69. 2%. The average Shannon index value within the species of F. engleriana was 0. 302 1, and the PPL within the population and among the nine populations was 72. 10% and 27. 90% respectively. The average Nei' s genetic diversity within the species of F. engleriana was 0. 204 5 , and 0. 150 2 for the index within the population. The coefficient of genetic differentiation was 0. 263 1. Compared with other species, the genetic diversity of F. engleriana was at a moderate level. And its genetic variance level within the populations was also at a moderate level.
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