补肾活血饮对帕金森病大鼠脑内多巴胺D2受体含量的影响

摘要

Objective To evaluate the correlation between single nudeotide polymorphisms (SNP) in the promoter of ataxia-telangiectasia mutated (ATM) gene and the susceptibility of nasopharyngeal carcinoma in Cantonese. Methods A total of 176 NPC Cantonese NPC patients and 202 age-matched healthy controls were enrolled in this study. G/A genotyping of the SNP locus rsl89037 in the promoter of ATM gene was performed by PCR and direct sequencing of the PCR products. Results The genotype frequency of heterozygote G/A was 50% (88/176) and 47.5% (96/202), and that of the homozygote variant A/A was 15.3% (27/176) and 18.3% (37/202) in NPC patients and healthy individuals, respectively. Statistics analysis revealed no evident correlations of the G/A and A/A genotypes to the clinical phenotypes of NPC in either NPC group or healthy control group (OR=1.022, 95%CI=0.668-1.564 for G/A + A/A). Analysis after stratification by age and gender found no such correlations either. Conclusion The genotype frequencies of SNP locus rsl89037 in the promoter of ATM gene are similar between NPC patients and healthy subjects, suggesting that rsl89037 is not related to the genetic susceptibility of NPC in Cantonese.%目的 探讨补肾活血饮治疗帕金森病(PD)的作用机制.方法 用直接向脑组织内注入6-羟基多巴损毁脑黑质致密部的方法建立PD大鼠模型.将120只SD大鼠随机分为正常对照组、生理盐水模型组和补肾活血饮治疗组,观察各组PD大鼠异常不自主运动变化；用放射免疫法测定治疗后大鼠脑组织多巴胺D2受体(DRD2)平衡解离常数(KD)和最大结合量(Bmax)的变化；免疫组化法观察脑组织DRD2受体阳性细胞数.结果 补肾活血饮组大鼠的异常不自主运动较模型组减少.补肾活血饮治疗组大鼠损毁侧脑组织DRD2 Bm较模型组显著增高(P＜0.01),KD值较模型组下降(P＜0.01)；DRD2受体阳性细胞数(80.9±13.59)较模型组(11.15±6.78)明显升高(P＜0.01),但与对照组无显著差异(P＞0.05).结论 补肾活血饮能改善PD大鼠不自主运动,促进PD模型大鼠脑组织DRD2表达,提高DRD2亲和力.