MACC1、HGF和C-met蛋白在卵巢上皮性癌中的表达及其意义

摘要

Objective To investigate the expressions of metastasis-associated in colon cancer-1 (MACC1), hepatocyte growth factor (HGF), and C-met proteins in epithelial ovarian cancer and their significance. Methods The expressions of MACC1, HGF and C-met in 20 specimens of normal ovarian tissues, 19 specimens of benign epithelial ovarian tumor and 52 specimens of epithelial ovarian cancer were measured by irnmunohistochemistry and Western blotting. The correlations of the expressions of MACC1, HGF and C-met protein to the clinicopathologic characteristics of epithelial ovarian cancer were analyzed, and the correlations between the expressions of the 3 proteins were also evaluated. Results The positivity rates of MACC1, HGF and C-met proteins were 73.1%, 63.5% and 78.8% in epithelial ovarian cancer with relative expressions of 0.72 ±0.05, 0.64 ±0.04 and 0.79 ±0.04, respectively, showing significant differences from those in normal ovarian tissues and benign ovarian tumors (P<0.05). In epithelial ovarian cancer, the up-regulation of MACC1, HGF and C-met expressions were associated with advanced FIGO stage, poor differentiation and lymph node metastasis (P<0.05). MACC1 expression was positively correlated to HGF (r=0.350, P= 0.011) and C-met expressions (r=0.429, p=0.002), and the latter two was also positively correlated (r=0.487, P=0.000). Conclusions MACC1 may serve as a potential biomarker for advanced ovarian cancer. Deregulation of MACC1, HGF and C-met proteins may synergistically participate in the malignant progression of epithelial ovarian cancer.%目的 探讨结肠癌转移相关基因1(metastasis-associated in colon cancer-1，MACC1)、肝细胞生长因子(HGF)和C-met蛋白在卵巢上皮性癌中的表达及其意义。方法采用免疫组织化学和Western blot技术检测20例正常卵巢组织、19例卵巢良性上皮性肿瘤组织和52例卵巢上皮性癌组织中MACCl、HGF和C-met蛋白的表达情况，分析三者与卵巢癌临床病理指标的关系及三者在卵巢癌组织中表达的相关性。结果MACC1、HGF和C-met蛋白在卵巢上皮性癌组织中的阳性率分别为73.1％、63.5％和178.8％，相对表达量分别为0.72±0.05、0.64±0.04和0.79±0.04，均显著高于正常卵巢和良性肿瘤组织(P＜0.05)。在卵巢上皮性癌中，MACCI、HGF和C-met异常高表达与临床分期、组织分化和淋巴结转移相关，临床分期越晚、组织分化越差、伴随淋巴结转移的癌组织中MACC1、HGF和C-met蛋白表达越高(P＜0.05)。卵巢上皮性癌中MACC1蛋白的表达与HGF和C-met蛋白的表达呈正相关(r=0.350，P=0.011;r=0.429，P=0.002)，HGF蛋白与C-met蛋白表达呈正相关(r=0.487,P=0.000)。结论 MACCl具有作为晚期卵巢癌分子标志物的潜在价值，MACC1、HGF和C-met异常可能协同参与卵巢上皮性癌的恶性进展。