Objective To investigate the role of Thl7 cells and the cytokine interleukin-17 (IL-17) in acute allograft rejection in mice. Methods Mouse models of kidney transplantation were randomly divided into rejection group and isograft group. On the post-operative day (POD) 3 and 7, we tested the serum IL-17 level using enzyme-linked immunosorbent assay and measured the number of Thl7 cells in the renal grafts by flow cytometry. The grafts were harvested and fixed in 10% formalin to prepare paraffin sections for routine pathological inspection. Results Compared to isograft group, the allograft group showed a significantly higher level of serum IL-17 on POD3 and POD7 (P<0.05), and the level of IL-17 is significantly higher on POD7 than on POD3 (P0<.05). The allograft group showed more infiltrating Thl7 cells in the grafts on POD3 and POD7 (P<0.05), and the cell number was significantly greater on POD7 (P<0.05). Pathological examination also showed an increased severity of graft rejection with the post-transplantation time. Conclusion Thl7 cells may play an important role in the development of renal graft rejection. IL-17 may serve as a potential specific indicator for predicting allograft rejection.%目的 研究Thl7细胞及相关因子白细胞介素17(IL-17)在小鼠肾移植排斥反应中的表达及意义.方法 建立小鼠肾移植模型,实验动物随机分为同系移植组和急性排斥反应组,在移植术后3d和7d,分别应用酶联免疫吸附实验检测两组小鼠血清中IL-17含量,应用流式细胞术检测移植肾中浸润淋巴细胞中Th17细胞数量,取移植肾经10％福尔马林固定后行常规病理检查.结果 与同系移植组相比,急性排斥反应组术后第3天及第7大血清中IL-17含量均升高(P＜0.05),且术后第7天时IL-17含量明显高于第3天(P＜0.05)；急性排斥反应组移植肾中浸润淋巴细胞中Th17细胞比例在术后第3天及第7天较『司系移植组均明显增多(P＜0.05),且术后第7大时Th17细胞比例明显高于第3天(P＜0.05)；病理组织学检查急性排斥反应组随着移植时间的延长,排斥反应逐渐增强.结论 Th17细胞在肾移植排斥反应发生发展中可能起着非常重要的作用,对移植脏器中IL-17的检测可作为急性排斥反应早期诊断的预见性和特异性指标.
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