早期肝癌血清蛋白质组标准模式图谱的建立及应用

摘要

目的 构建早期肝癌血清蛋白质组标准模式图谱,初步探讨其在肝癌早期诊断中的临床意义.方法 (1)收集正常人、早期肝癌患者血清各12例,分别等量混匀为两组样本,去掉血清中的白蛋白和IgG.取血清各300μg与水化液充分混合,双向凝胶电泳,实验重复3次,银染,图像分析,筛选差异表达的蛋白点,构建早期肝癌血清蛋白质组标准模式图谱.(2)应用该模式图谱盲法对13例慢性肝脏疾病患者和10名健康人进行验证.结果 筛选出两组间差异表达的蛋白点共38个,对表达量差异5倍以上的11个蛋白点进行分析,构建早期肝癌血清蛋白质组标准模式图谱.盲法分析示敏感性为92%,特异性90%.结论 早期肝癌患者血清2-DE蛋白质图谱与健康人有一定差异,其差异表达的蛋白质及其特定组合构成有望成为早期肝癌诊断与判定预后的模式图谱.%Objective To investigate the expression of RUNX3 protein in hepatic cell carcinoma (HCC) and its relationship with the clinicopathological factors of HCC. Methods Immunohistochemistry was performed to detect the expression of RUNX3 protein in HCC and the surrounding normal tissues, and the relation of RUNX3 with the clinicopathological factors of HCC was analyzed. Results The positivity rate of RUNX3 protein expression was 49.02% (25/51) in HCC tissues, significantly lower than that in the surrounding normal tissues [82.35% (42/51), x2=12.5706, P< 0.01). RUNX3 protein expression varied significantly with such pathological factors as the differentiation degree, cancer-associated thrombosis in the portal vein and intrahepatic metastasis (P＜0.05), but not with tumor diameter, location, tumoral hemorrhage, necrosis or histotypes of the tumor (P>0.05). Conclusion RUNIX3 protein expression is lowered in HCC as compared with that in the surrounding normal tissue, suggesting an important role of RUNX3 in the tumorigenesis and development of HCC and the possible identity of RUNX3 gene as an anti-oncogene of HCC.