血管内皮细胞生长因子在重症急性胰腺炎大鼠中的表达及作用

摘要

Objective To investigate the role of vascular endothelial growth factor (VEGF) in the pathogenesis of severe acute pancreatitis (SAP) in rats. Methods Sixty-four male SD rats were randomly divided into control group and SAP group, and in the latter group, SAP was induced by retrograde injection of 5% sodium taurocholate in the pancreaticobiliary duct. The rats were sacrificed at 1, 3, 6 and 12 h after the operation, and the severity of pancreatitis was assessed according to histological scoring. The serum levels of VEGF were examined with enzyme-linked immunosorbent assay, and the expression of VEGF in the pancreatic tissues was measured by SP immunohistochemistry. Another 30 SD rats were randomized into the control group, SAP group and SAP + recombinant rat VEGF injection group, and the vascular permeability of the pancreatic microcirculation was determined by Evans Blue leakage test. Results At each of the time points for measurement, both the serum VEGF level and scores of pancreatic tissue injury were significantly higher in SAP group than in the control group (P<0.05). Compared with the control group, the expressions of VEGF in the pancreatic tissues of SAP group were significantly up-regulated following the operation (P<0.05). The vascular permeability of the pancreatic microcirculation significantly increased after the onset of SAP, and injection of recombinant rat VEGF significantly increased the leakage rate of Evans Blue. Conclusion VEGF may play an important role in the pathogenesis of pancreatitis and in causing edema and hemorrhage in SAP, and the level of serum VEGF may reflect the severity of pancreatic injury.%目的 观察血管内皮细胞生长因子(VEGF)在大鼠重症急性胰腺炎(SAP)中的作用,并探讨其可能机制.方法 SD大鼠64只,5%牛磺胆酸钠逆行胆胰管注射制备SAP模型,分别于术后1、3、6、12 h处死动物,观察各组大鼠胰腺组织形态学改变,免疫组化检测VEGF在各组大鼠胰腺组织中的表达,ELISA法测定血清VEGF活性.另取SD大鼠30只,随机分为对照组、SAP组及SAP+VEGF重组体注射组,每组各10只,应用伊文思蓝漏出率法检测胰腺微循环血管通透性.结果 重症急性胰腺炎大鼠胰腺组织中VEGF较对照组明显升高(P＜0.05),血清VEGF含量显著升高(P＜0.05),并随SAP时间延长而逐渐升高(P＜0.05).SAP时胰腺血管通透性显著升高(P＜0.05),应用VEGF重组体注射后伊文思蓝漏出率升高显著(P＜0.05).结论 VEGF参与了SAP的发病过程,可能是胰腺炎时水肿充血的重要原因之一,血清VEGF水平可在一定程度上反映胰腺病理损伤程度.