过氧化物酶1,6和GFAP在人脑星形胶质细胞瘤中的表达及临床意义

摘要

目的 确定星形胶质细胞瘤中过氧化物酶1(Prxl)、过氧化物酶6(Prx6)和胶质纤维酸性蛋白(GFAP)的表达情况,探讨其表达水平与星形胶质细胞瘤恶性程度的关系.方法 采用Western blot、RT-PCR和免疫组化检测52例星形胶质细胞瘤(Ⅱ级23例、Ⅲ级15例、Ⅳ级14例)和12例正常脑组织标本中Prx1、Prx6和GFAP的表达情况.结果 Prx1、Prx6在正常脑组织、Ⅱ级、Ⅲ级、Ⅳ级星形胶质细胞瘤中蛋白和mRNA的表达水平逐渐升高,具有统计学意义(P＜0.05)；GFAP在Ⅲ、Ⅳ级星形胶质细胞瘤中蛋白和mRNA的表达水平低于Ⅱ级星形胶质细胞瘤和正常脑组织(P＜0.05).结论 Prx1、Prx6和GFAP可能是临床评价星形胶质细胞瘤恶性程度和侵袭性的潜在生物标志物,可作为星形胶质细胞瘤生物治疗的潜在靶分子.%Objective To characterize the expressions of peroxiredoxin 1 (Prx1), peroxiredoxin 6 (Prx6) and glial fibrillary acidic protein (GFAP) in human brain astrocytoma and explore their clinical significance. Methods The protein and mRNA expression levels of Prxl, Prx6 and GFAP in human brain astrocytoma and normal brain tissue specimens were determined by Western blotting, RT-PCR and immunohistochemistry. Results The protein and mRNA expressions of Prxl and Prx6 increased significantly in the order of normal brain tissue, grade II astrocytoma, grade III astrocytoma and grade IV astrocytoma (P< 0.05). The protein and mRNA expressions of GFAP decreased significantly in grade III and IV astrocytoma compared with those in grade II astrocytoma and normal brain tissues (P<0.05). Conclusion Prxl and Prx6 may play important roles in the invasion and malignant development of human brain astrocytoma, and may serve as biomarkers for evaluating the invasiveness, malignancy and prognosis of the tumor as well as potential molecular targets in astrocytoma therapy.