高强度聚焦超声联合吉西他滨对裸鼠胰腺癌皮下移植瘤的疗效

摘要

目的：通过高强度聚焦超声（HIFU）及化疗药物（吉西他滨）作用于人胰腺癌裸鼠皮下移植瘤，评估HIFU及化疗对异位移植瘤的疗效。方法构建人胰腺癌裸鼠皮下移植瘤模型，设定为HIFU组，化疗组，HIFU联合化疗组及对照组，治疗后每周测量肿瘤长径、短径、共观察5周，绘制肿瘤曲线，计算抑瘤率，并通过免疫组化检测瘤组织中血管内皮生长因子的表达水平。结果各治疗组肿瘤体积差异显著（P<0.01），均明显小于对照组，差异显著（P<0.01），差异最显著为对照组与联合治疗组之间（P<0.001）。HIFU联合化疗组抑瘤率最明显，与单化疗及单HIFU组差异显著。血管内皮细胞生长因子表达在联合组水平最低，对照组水平最高。结论HIFU治疗胰腺癌异位移植瘤疗效确切，能明显延缓肿瘤进展，HIFU联合化疗为最佳治疗模式。%Objective To investigate the effect of high-intensity focused ultrasound (HIFU) and gemcitabine on xenograft growth in nude mice bearing human pancreatic cancer. Methods Nude mouse models bearing subcutaneous human pancreatic cancer cell line PANC-1 xenograft were randomized into 4 groups, including a control group and 3 treatment groups subjected to treatments with HIFU, gemcitabine, or both. After the treatments, the tumor was measured on a weekly basis for 5 weeks, and the tumor growth curve was drawn. The tumor inhibition rate was calculated and the expression of vascular endothelial growth factor (VEGF) in the tumor tissue was examined by immunohistochemistry. Results The tumor volume showed significant differences between the 3 treatment groups (P<0.01), but all significantly smaller than that in the control group; HIFU combined with gemcitabine resulted in the most obvious reduction in the tumor volume. VEGF expression in the tumor tissue was the lowest in the combined treatment group and the highest in the control group. Conclusion HIFU therapy produces definite therapeutic effect on human pancreatic cancer in the nude mouse model, and its combination with chemotherapy is the optimal treatment modality.