放疗联合高强度聚焦超声治疗裸鼠胰腺癌的疗效分析

摘要

Objective:To analyze the effect of high intensity focused ultrasound (HIFU) combined with sequential radiotherapy on pancreatic cancer via transplanted tumor mice model.Methods:PANC-1 cells were injected subcutaneously into the right scapular region of twenty-four BALB/c nu-nu mice.The mice were then randomly divided to 4 groups, control group, HIFU alone group, RT alone group, HIFU combined RT group.The tumor volume change and acute side reaction were measured.The expressions of Ki-67, Caspase-3, STAT-3, and HMGB-1 were analyzed using immunohistochemistry.Results:In the control group, tumor volume increased significantly.Tumor control rate was 36.32% in HIFU alone group, 84.49% in RT alone group, and 70.49% in HIFU combined RT group, respectively (P<0.05).There was no acute adverse event in control and RT alone groups.Two shock reaction and four cases of burn injury reaction were all found in HIFU and HIFU combined RT groups.The expression of HMGB-1 was significantly increased in HIFU and HIFU combined RT groups as compared with control and RT alone groups (P<0.05).Ki-67 and STAT-3 were highly expressed in all groups, but the expression of Caspase-3 was quite low in all groups.Conclusions:The treatment of HIFU combined with sequential radiotherapy on pancreatic cancer failed to show synergistic effect, and the adverse reaction was higher than that of radiotherapy alone.%目的:利用裸鼠移植瘤模拟胰腺癌模型探讨放疗联合高强度聚焦超声(HIFU)治疗裸鼠胰腺癌的疗效.方法:将人胰腺癌细胞系PANC-1接种于24只裸鼠并成功建立移植瘤模型,随机分为4组:空白对照组,HIFU组,放疗组(10 Gy×3次),HIFU+放疗组.观察肿瘤体积变化、治疗急性损伤情况,再取肿瘤标本用免疫组化方法检测Ki-67、Caspase-3、STAT-3、HMGB-1的表达情况.结果:空白对照组肿瘤体积明显增大,HIFU组、放疗组、放疗联合HIFU组肿瘤抑制率分别为36.32%、84.49%、70.49%,组间差异有统计学意义(P<0.05).放疗组与对照组无急性治疗损伤,HIFU组与HIFU+放疗组各有2例休克反应及4例皮肤烫伤反应.HMGB-1表达在HIFU组和HIFU+放疗组明显高于其他两组(P<0.05),放疗组与空白对照组未见差异.Ki-67与STAT-3在各组均为高表达,Caspase-3在各组均低表达.结论:HIFU联合放射治疗胰腺癌裸鼠皮下瘤未显示协同效应,且不良反应高于单独放疗.