目的：利用蛋白质组学方法研究慢性乙型肝炎肝郁脾虚、湿热中阻同病异证病人外周血总蛋白表达谱的变化，分析差异表达蛋白质与证型之间的关系。方法通过双向凝胶电泳，获得了外周血浆差异蛋白表达图谱，经基质辅助激光解析飞行时间质谱分析鉴定出差异表达的蛋白质。结果获得了慢性乙型肝炎中医肝郁脾虚和湿热中阻同病异证病人及健康人对照组血浆总蛋白双向凝胶电泳银染图谱，健康组蛋白点为278±16个，肝郁脾虚证组为320±14个，湿热中阻证组为343±19个；通过对差异蛋白质点进行质谱鉴定，共发现7个差异表达蛋白，主要包括免疫相关蛋白，炎症蛋白以及脂类代谢相关蛋白。结论慢性乙肝肝郁脾虚和湿热中阻证型间存在多个差异表达蛋白，提示不同的蛋白质表达谱可能是同病异证的分子标签和证侯基础。%Objective To analyze the differential proteomic profiles of the plasma in patients with chronic hepatitis B presenting with damp-heat retention syndrome and liver stagnation-spleen deficiency syndrome. Methods Two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify the differentially expressed proteins in the plasma of patients with chronic hepatitis B. Results The numbers of plasma proteins detected in healthy volunteers, patients with damp-heat retention syndrome, and patients with liver stagnation-spleen deficiency syndrome were 278 ± 16, 320 ± 14 and 343 ± 19, respectively. Seven differential protein spots were successfully identified by mass spectrum, and were classified into immunological proteins, inflammatory proteins, and lipid metabolism-related proteins. Conclusion In patients with chronic hepatitis B, the multiple differential proteins between damp-heat retention syndrome and liver stagnation-spleen deficiency syndrome suggests the diverse molecular basis of traditional Chinese medicine syndromes, and they might be the molecular tags for different syndromes of the same disease.
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