超声辐射力结合靶向超声微泡评价小鼠肾缺血再灌注损伤

摘要

Objective To study the sensitivity and feasibility of detecting microvascular inflammation in renal ischemiareperfusion injury using microbubbles (MB) targeted to the intercellular adhesion molecule ICAM-1 and ultrasound radiation force (USRF).Methods Mouse models of kidney ischemia-reperfusion were randomized into 5 groups with reperfusion time of 1,3,6,12,and 24 h (IRlh,IR3h,IR6h,IR12h,and IR24h group,respectively).Each group was subdivided into targeted MB group (MBIcAM group) and targeted MB +USRF group (MBICAM+USRF group).Kidney enhancement and the video intensity (VI) of the kidneys were compared among the groups.Results In normal mice in either MBICAM or MBICAM +USRF group,no obvious enhancement of the kidney or significant increase in VI of the kidneys was observed (P=0.923).The kidneys were enhanced in all the mice with renal ischemia-reperfusion injury,and with the passage of time,the enhancement increased progressively.VI in the kidneys of mice with renal ischemia-reperfusion injury in MBICAM+USRF group increased more significantly compared with the MBICAM group.Significant difference in the VI was noted among the groups with different perfusion time but not between IR12h and IR24h groups.Conclusion Microbubbles targeted to ICAM-1 combined with USRF can effectively evaluate renal ischemia-reperfusion injury in mice and can be used for early evaluation of microvascular inflammation and other endothelial responses.%目的 探讨超声辐射力促进靶向微泡粘附提高肾缺血再灌注(IR)损伤检测的敏感性及可行性.方法 制作小鼠肾缺血再灌注模型及正常对照组,并根据再灌注后1、3、6、12、24 h分别分为IR1h、IR3 h、IR6 h、IR12h、IR24 h组.各组再根据有无超声辐射力作用随机分为单击靶向微泡(MBICAM)组和靶向微泡+超声辐射力组(MBICAM+USRF)组.结果 MBICAM组和MBICAM+USRF组在对照组肾脏均未见明显显影增强,肾脏声强度(VI)值无显著差异(6.47±0.42对6.45±0.62,P=0.923).肾脏IR损伤不同时间窗下均可见显影增强,并且随着时间的推移,显影强度逐渐增高.MBICAM+USRF组缺血再灌注各时间窗肾脏VI值较MBICAM组显著增大,两者之间均存在显著性差异(P均=0.000).IR12 h和IR24 hVI值之间无显著差异(P=1.000),其余各组之间均存在显著差异(P＜0.05).结论 应用超声辐射力联合携抗细胞间黏附分子1单抗靶向微泡可提高小鼠肾脏缺血再灌注损伤检测敏感性,可用于早期评价微血管炎症或相关的血管内皮反应.