[Objective]An easy and efficient process to obtain mangiferin from mango leaves was set up to provide raw materials and technical support for developing mangiferin products.[Method]Through five steps(material crushing→ethylacetate(EAC)impurity-removing→methanol(Met)extraction→equivalent extract washing→crystal precipitation),mangiferin was isolated from mango leaves.By means of single factor test and L9(34)orthogonal test,the technical param-eters including extraction time,ultrasonic power,extraction frequency and extraction temperatue were optimized.[Result]The optimized process for extracting mangiferin from mango leaves by EAC impurity-removing method was as follows:mango leaves were crushed and sifted,added 3 mL/g EAC,soaked for 4 h in sealed container,extracted by ultrasonic vi-brating for 20 min(50℃,350 W),filtrated through 100 mesh for 3 times at room temperature;added 100% Met in the residue on the basis of 3 mL/g,extracted by ultrasonic vibrating(20 min,50℃,350 W),filtrated through 100 mesh while its was hot;mixed the extractions,condensed by vacuum decompression to get extractum with 25.5% mangiferin;added 100% Met in the extractum on the basis of 4 mL/g,stirred washing for 5 min at room temperature,let it still for 10 min,fil-trated through 100 mesh for 3 times,dried the residue at 60℃to get the crude product;added 100% Met in the crude prod-uct on the basis of 4 mL/g,stirred washing for 5 min at 50℃,put it still for 8 h at 6℃,dried the residue at 60℃,crystal-lized for twice to obtain pure product with purity>98.00% and recovery rate 79.10%.Using this process,crude mangifer-in product and pure mangiferin product could obtain.The purity of crude mangiferin product>64.00% with recovery rate of 83.90%,and purity of pure mangiferin product >98.00% with recovery rate of 66.40.[Conclusion]The optimized EAC impurity-removing process is an easy,low-cost and efficient operation to extract mangiferin from mango leaves,and can be applied in real mangiferin production.%[目的]优化酯提除杂法提取分离芒果叶芒果苷工艺,为芒果苷相关产品的开发利用提供原料和技术支持.[方法]采用原料粉碎→酯提除杂→甲醇提取→浓浸膏洗涤→结晶沉淀的五步法从芒果叶中提取分离芒果苷,通过单因素试验和L9(34)正交试验对提取时间?超声波功率?提取次数和提取温度等工艺参数进行优化.[结果]酯提除杂法提取分离芒果叶中芒果苷的最佳工艺:将芒果叶粉碎过筛;按3mL/g加入乙酸乙酯,密封浸泡4h,超声波振荡提取20min(50℃,350W),室温100目过滤,反复提取3次;在残渣中按3mL/g加入100%甲醇,超声波振荡提取4次(20min,350W,55℃),趁热100目抽滤;将各次提取液合并,真空减压浓缩得浓浸膏产品;按4mL/g加入100%甲醇,室温搅拌洗涤5min,静置10min,100目过滤,反复洗涤3次,滤渣60℃干燥得芒果苷粗品;按4mL/g加入100%甲醇,50℃搅拌洗涤5min,6℃静置8h,取出过滤,滤渣60℃干燥,反复结晶2次.按照上述工艺可获得芒果苷粗品和纯品2种产品,粗品的芒果苷纯度>64.00%,总回收率83.90%,纯品的芒果苷纯度>98.00%,总回收率约66.40%.[结论]优化后的酯提除杂法提取分离芒果苷工艺操作简便?成本低廉?效率高,可用于芒果苷实际生产.
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