神阙灸法治疗EAT模型小鼠的疗效及机制初探

摘要

Objective:To investigate the effect and possible mechanism of Shenque ( CV8) moxibustion therapy on the mice with autoimmune thyroiditis .Methods:Experimental autoimmune thyroiditis ( EAT) model was replicated on mice with high iodine intake and subcutaneous immunization of porcine thyroglobulin ( PTg ) .The mice were randomly divided into blank group , EAT model group and treatment group .The thyroid stimulating hormone ( TSH) concentrations and thyroglobulin antibody ( TGAb ) concentrations were detected by enzyme-linked immunosorbent assay ( ELISA) .The morphological changes of thyroids and local skin tissues around CV 8 were observed by HE staining .The expression of cytokine transforming growth factor-β1 ( TGF-β1 ) and transient receptor potential va-nilloid subfamily member 1 ( TRPV1) of thyroid tissues and local skin tissues around CV 8 were detected by immu-nohistochemistry (IHC).Results:Comparing with the EAT model group , the mice from treatment group presented lighter congestion , swollen and inflammatory cells infiltration , degeneration and necrosis of follicular epithelial cells in thyroid tissues , along with normal production of thyroglobulin .The expression of TGF-β1 and TRPV1 in the local skin tissues around CV 8 were enhanced significantly after treatment ( P<0 .05 ) , however , there were no significant changes in the thyroid tissues ( P>0 .05 ) .The serum TGAb concentrations of treatment group were significantly lower comparing with the EAT model group ( P<0 .05 ) , however , there was no significant differences in the serum TSH concentrations among groups ( P>0 .05 ) .Conclusion: Using Moxibustion therapy on CV 8 can significantly ease the inflammation in the thyroid tissues of EAT mice .The up regulation of TGF-β1 and TRPV1 in the local skin tissues around CV8 after moxibustion therapy may suppress the immune response , moreover, causing a down regula-tion of thyroid autoantibody .%目的:观察神阙灸法对实验性自身免疫性甲状腺炎(EAT)模型小鼠的疗效并探讨可能机制.方法:运用猪甲状腺球蛋白(PTg)皮下多点免疫联合饮用高碘水的方法制作小鼠EAT模型,并随机分为空白组、模型组、治疗组.采用酶联免疫吸附法(ELISA)检测小鼠促甲状腺素(TSH)、甲状腺球蛋白抗体(TGAb)水平;苏木素-伊红染色(HE)观察甲状腺及神阙穴局部皮肤组织形态;免疫组织化学法检测甲状腺、神阙穴局部皮肤组织中的转化生长因子-β1(TGF-β1)、瞬时受体电位香草酸亚家族-1(TRPV1)水平.结果:与模型组相比,治疗组小鼠甲状腺组织充血、水肿、滤泡上皮变性坏死和炎症细胞浸润均较轻,甲状腺球蛋白合成正常,且小鼠血清TGAb水平显著下降(P<0.05),神阙穴局部皮肤组织TGF-β1、TRPV1表达增强(P<0.05),而血清TSH、甲状腺组织TGF-β1、TRPV1水平未见显著差异(P>0.05).结论:神阙灸法能够显著减轻EAT小鼠甲状腺组织炎症反应,其疗效可能与神阙灸法激活局部组织TGF-β1、TRPV1表达,继而抑制免疫反应,使EAT小鼠甲状腺自身抗体水平下降有关.