三七总皂苷联合顺铂对肝癌细胞HepG2和SMMC-7721增殖、迁移、侵袭及凋亡的影响

摘要

目的:探讨三七总皂苷(PNS)联合顺铂(DDP)对肝癌细胞HepG2和SMMC-7721增殖、迁移、侵袭及凋亡的影响及其可能机制.方法:采用四甲基偶氮唑盐比色法(MTT法)检测不同浓度PNS和DDP分别及特定浓度联合处理对肝癌细胞HepG2和SMMC-7721增殖的作用,划痕实验检测肝癌细胞迁移的改变,Transwell小室实验检测肝癌细胞侵袭的改变,流式细胞术检测其对肝癌细胞凋亡的影响,蛋白质印迹法测定蛋白表达水平的改变.结果:应用PNS和DDP均能抑制肝癌细胞的增殖,呈现剂量-效应关系;PNS(200 μmol·L-1)联合顺铂(5 μmol·L-1)可显著抑制肝癌细胞增殖、迁移及侵袭的能力,增加细胞凋亡率;两者联合可降低肝癌细胞抗凋亡蛋白Bcl-2的表达,增加凋亡蛋白Bax的表达.结论:PNS联合DDP可显著抑制肝癌细胞HepG2和SMMC-7721的增殖,且能显著降低肝癌细胞迁移和侵袭的能力,增加肝癌细胞的凋亡.%Objective: To investigate the effects and mechanism of Panax notoginseng saponins(PNS) in combination with cisplatin(DDP) on the HepG2 and SMMC-7721 cells.Methods: The cellular proliferation of HepG2 and SMMC-7721 cells treated with PNS and DDP at the indicated concentrations was detected by MTT assay.The migration and invasion of HCC cells were detected by wound healing assay and matrigel invasion assay.Flow cytometry assay was used to analyze the changes of cell apoptosis.The protein expression was measured by western blot assay.Results: PNS and DDP inhibited cell viability of HCC cells in a dose-dependent manner.PNS combined with DDP significantly suppressed the cell growth, migration and invasion of HCC cells.Besides, PNS in combination with DDP could significantly decrease the expression of Bcl-2 and increase the expression of Bax to induce the apoptosis of HCC cells.Conclusion: PNS in combination with DDP can inhibit cell growth, migration and invasion and promote the apoptosis of HCC cells.