IGF-1对多发性抽动症模型大鼠纹状体神经元氨基酸递质水平及细胞凋亡的影响

摘要

目的:研究胰岛素样生长因子-1(IGF-1)对多发性抽动症模型大鼠纹状体神经元内氨基酸递质水平及细胞凋亡的影响.方法:将24只Wistar大鼠随机均分为空白对照组、模型组(亚氨基二丙腈诱导建立)及治疗组(建模成功后给予IGF-1) .采用刻板运动评分进行行为学检测,高效液相色谱法法测定纹状体内氨基酸递质谷氨酸(Glu)和γ-氨基丁酸( GABA)的浓度,蛋白印迹法检测纹状体组织凋亡相关因子核转录因子-κB(NF-κB) 、半胱天冬酶-3( caspase-3)的表达情况.结果:与对照组比较,模型组大鼠刻板运动评分显著增加,纹状体内Glu和GABA含量明显上升(P<0.05);模型组大鼠纹状体组织NF-κB、caspase-3蛋白表达明显多于对照组(P<0.05) .与模型组比较,治疗组大鼠经IGF-1处理后,刻板运动评分、纹状体内Glu和GABA水平均明显减小,NF-κB、caspase-3蛋白显著下调(均P<0.05) .结论:IGF-1可能通过抑制纹状体内氨基酸递质水平及凋亡蛋白表达.对多发性抽动症大鼠纹状体具有一定的保护作用.%Objective: To study the effects of insulin-like growth factor-1(IGF-1) on striatal amino acid transmit-ter and cell apoptosis in rat models of tourette syndrome model.Methods: Twenty-four Wistar rats were randomly divided into three groups: control group, model group(induced by 3, 3-iminodipropionitrile, IDPN), and treat-ment group( rats injected with IGF-1 after given with IDPN).The protective effects of IGF-1 were estimated by ob-serving the rigid motion score.Glutamic acid(Glu) and γ-aminobutyric acid(GABA) in the rat striatum were de-tected by high performance liquid chromatography method.The apoptotic protein NF-κB and caspase-3 in striatal tissue were detected by Western blotting.Results: Compared with the control group , rigid motion score, levels of striatal Glu and GABA in the model group were significantly increased (P<0.05).The protein levels of caspase-3 and NF-κB in model group were higher than those in the control group ( P<0.05 ).Compared with the model group, after IGF-1 treatment, rigid motion score, levels of striatal Glu and GABA in the treatment group were sig-nificantly decreased (P<0.05), the protein levels of caspase-3 and NF-κB in the treatment group were dramatical-ly down-regulated ( P<0.05).Conclusion: IGF-1 has certain neuroprotective effects on striatal neurons in rat model of Tourette syndrome.Its mechanism may be related to the inhibition of striatal amino acid transmitter and apoptotic protein expression.