2株红树内生真菌混合发酵培养液中代谢产物的分离鉴定

摘要

Mixed fermentation technique was applied to culture two marine derived mangrove endophytic rnfungi K38 and E33 from the South China Sea coast. Five secondary metabolites were isolated from the rncrude extracts. Their structures were elucidated by spectral methods as 2-formyl-3,5-dihydroxy-4-methylrnbenzoic acid ( compound 1 ), 6,8-dihydroxy-4-acetylisocoumarin ( compound 2 ), 5-hydroxy-6,8-dimernthoxy-2,3-dimethyl-4H-naphtho- [ 2,3-b ] -pyran-4-one ( compound 3 ), 7,22- ( E ) -diene-3β, 5 α, 6β-triolrnergosta( compound 4) and mannitol (compound 5). Among them, there were no previous report about rncompounds 1,2 and 3 isolated from marine fungi.%将混合发酵技术应用于南海红树林内生真菌E33和K38代谢产物的培养,从该提取物中分离到5个化合物,利用波谱技术确定其结构分别为2-甲酰基一3,5-二羟基-4-甲基苯甲酸(化合物1)、6,8-二羟基-4-乙酰基异香豆素(化合物2)、5-羟基-6,8-二甲氧基-2,3-二甲基-4H-萘-[2,3-b]-吡喃-4-酮(化合物3)、7,22-(E)-二烯-3,5,6-三羟基麦角甾醇(化合物4)、甘露醇(化合物5).其中,化合物1、2和3以往鲜见从海洋真菌中分离到的报道.