猪源狂犬病病毒N和G基因的序列分析

摘要

通过对新分离的猪源狂犬病病毒GD-SH01株N基因和G基因进行序列分析,解析它们与其他狂犬病病毒N和G基因的差异.提取猪源狂犬病病毒GD-SH01株的总RNA,采用RT-PCR的方法扩增N基因和G基因,并与T载体连接,克隆至大肠埃希菌DH5α.测序后与国内外部分毒株的N基因和G基因进行核苷酸序列和氨基酸序列比对.与其他毒株相比,N基因核苷酸相似性为84.3％ ～ 98.0％；氨基酸相似性为92.5％ ～ 99.3％.G基因核苷酸相似性为80.4％ ～ 98.2％；氨基酸相似性为87.8％ ～ 99.6％.%To understand the difference of the nucleoprotein (N) gene and glycoprotein (G) gene between the strain of pig origin and other rabies viruses. The N and G genes of rabies virus isolated from the brain of a rabid pig in Guangdong Province were amplified by RT-PCR, cloned, sequenced and compared with the genes of other rabies virus. The results showed that the sequence of nucleotides and amino acids of N genes of GD-SH01 shared 84. 3% -98. 0% and 92. 5% -99. 3% similarity, and G genes of GD-SH01 shared 80. 4% -98. 2% and 87. 8% -99. 6% similarity, respectively, compared to other viruses.