Investigation of Surface Water for Contamination with Pseudorabies Virus in Runoff from Pseudorabies Quarantined Nebraska Swine Poduction Areas

摘要

The objective was to develop methods to concentrate water samples and quantitate virus and to apply the techniques to virological assay of storage ponds, lagoons, waste disposal sites and surface water runoff from swine production areas for contamination with pseudorabies virus. Molecular filtration has been used to concentrate pseudorabies virus (PRV; Herpevirus suis) from six liters of saline or water. Essentially all of the PRV added to the six liters of fluid was concentrated in 100 ml of filtration retentate. Inclusion of a cellulose acetate filter in the system effectively removed particulate matter without reducing the viral titer of the filtrate. The ability to remove particulate matter from water renders the system applicable to concentration of surface water runoff for assay for the presence of PRV.