低温对不同冷敏感型甘蔗品种根系一些生理旨标的影响

摘要

为了探明低温对不同冷敏感型甘蔗品种根系一些生理指标的影响,本研究以不同冷敏感型甘蔗桂糖28号(抗冷型)和园林6号(冷敏感型)为供试材料,研究低温胁迫后根部的根系活力、Ca2+-ATP酶活性、电导率以及丙二醛、脯氨酸含量等生理指标.结果表明:随低温胁迫时间的延长,2个品种根系活力都呈现下降趋势,桂糖28号的根系活力均显著大于园林6号；低温胁迫后2个品种相对电导率都呈现上升趋势,但桂糖28号上升较平缓,而园林6号电导率上升速度较快,园林6号电导率均大于桂糖28号；低温胁迫后2个品种丙二醛含量都呈现上升趋势,园林6号丙二醛含量显著大于桂糖28号；低温胁迫后2个品种的脯氨酸含量都呈现上升趋势,桂糖28号脯氨酸含量均显著高于园林6号:低温胁迫后,桂糖28号Ca2+-ATP酶活性呈先上升再下降趋势,而园林6号Ca2+-ATP酶活性一直呈下降趋势,桂糖28号Ca2+-ATP酶活性均显著高于园林6号.可见,在低温胁迫下,具较高的根系活力、Ca2+-ATP酶活性及脯氨酸含量可能是耐冷型甘蔗品种桂糖28号具有更强抵御低温胁迫能力的生理基础.%In order to study the effect of low temperature on some physiologic indices of sugarcane root system, two sugarcane varieties GT28(cold resistant) and YL6(cold sensitive) with 9 to 10 leaves were put into 0 ℃ biotron and the sugarcane root system samples were taken after 0,2,4 and 6 d respectively to determine the root activity, conductivity percentage, Ca2 + -ATPase activity, MDA and proline content. The results were as follows: After low temperature stress, the root activity of two sugarcane varieties decreased with the extension of time, the root activity of GT28 was higher than that of YL6. After low temperature stress, the conductivity percentage of two sugarcane varieties increased with the extension of time, the conductivity percentage of YL6 was higher than that of GT28. Under low temperature stress, the proline content and MDA content of two sugarcane varieties increaed markedly with the extension oftime, the MDA content of YL6 was higher than that of GT28, and the proline content of GT28 was higher than that of YL6. The Ca2 + -ATPase activity of GT28 increased at first, then decreased, and the Ca +-ATPase activity of YL6 decreased gradually all the time, the Ca2 + -ATPase activity of GT28 was higher than that of YL6. It might be concluded that under the similar chilly stress, the chilly resistance cultivar could retain a higher root activity, Ca2 + -ATPase activity, and proline content.