用于乙醇生产的‘热研4号’王草石灰预处理条件优化研究

摘要

Objective] The pretreatment of Pennisetum purpureum ×P.americanum cv.Reyan No.4 was studied with lime to improve the conversion rate for ethanol production .[Method] The effects of pretreat-ment time , temperature , the lime dosage , and solid-to-liquid ratio as well as the orthogonal optimization test on the composition and the following enzymatic hydrolysis of ‘Reyan No.4’ were investigated.[Re-sult and conclusion] The optimal pretreatment conditions were as follows:pretreatment time 3 h, temper-ature 125℃, solid-to-liquid ratio 1∶12 ( g· mL-1 ) and the lime dosage of material 0.15 g· g-1 .Under the optimal condition, dry mass loss was 28.1% in lime pretreatment, and the removal rates of lignin, hemicellulose and cellulose were 65.4%, 37.5%, 6.1%, respectively .The yields of hemicellulose , cellulose and total residue were 45.5%, 85.7%, 55.2%, respectively; in the following enzymatic hy-drolysis, they increased 18.1 times, 4.5 times and 4.4 times, respectively, compared with those of un-treated ‘Reyan No.4’ .The primary factors influencing enzymatic hydrolysis yield were temperature and time, followed by lime dosage and solid-to-liquid ratio.%【目的】提高‘热研4号’王草Pennisetum purpureum ×P．americanum cv．Reyan No．4生产乙醇的转化率．【方法】采用石灰进行预处理，对时间、温度、石灰用量、固液比4个因素进行单因素和正交优化试验分析．【结果和结论】最优预处理条件为：时间3 h，温度125℃，原料的石灰用量0.15 g· g-1，固液比（m∶V）1∶12（g· mL-1）．此时‘热研4号’王草的干基失质量率、木质素移除率、半纤维素移除率和纤维素移除率分别为28.1％、65.4％、37.5％和6.1％．预处理的‘热研4号’王草经酶解后，半纤维素、纤维素和总酶解得率分别为43.5％、85.7％和55.2％，比未经处理的酶解得率分别提高了18.1、4.5和4.4倍．此外，影响酶解得率的主要因素是预处理时间和温度，其次是石灰用量和固液比．