目的：探究GS H对重型儿童哮喘气道巨噬细胞凋亡的影响。方法通过 GS H 检测试剂盒检测重型及轻型哮喘患儿巨噬细胞 AM 内的GSSG 水平，并采用 MTT 法检测巨噬细胞 AM 的细胞活力，Western blot 法检测凋亡相关蛋白 Cleaved caspase 3的表达情况。结果（1）重型哮喘患儿的吞噬细胞内 GSSG 水平显著高于轻型患儿；（2）哮喘患儿巨噬细胞 AW 的细胞活力与轻型哮喘患儿相比显著降低，且体外补充的GSH可以逆转细胞活力降低；（3）体外补充GSH 可以逆转重型哮喘患儿巨噬细胞凋亡蛋白Cleaved caspase 3的表达增多。结论体外补充GS H 可以抑制重型哮喘患儿气道巨噬细胞凋亡，发挥保护作用。%Objective To observe the effects of glutathione(GSH) on apoptosis of airway macrophage(AM) in children with severe asthma . Methods The GSSG level of AM in children with severe/moderate asthma was de‐termined by GSH assay kit .MTT was used to analyze AM cell viability .The apoptotsis protein expressions of cleaved caspase 3 was detected by Western blotting in vitro . Results The GSSG level of AM in children with se‐vere asthma was significantly higher and the cell viability was significantly lower than the moderate .The apoptosis expressions of cleaved caspase 3 of AM in children with severe asthma was significantly increased than the moder‐ate ,which was reversed by 500 μM GSH in vitro . Conclusion In vitro ,GSH supplement can inhibit apoptosis and rescue AM in children with severe asthma .
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