共振光散射法在核酸测定中的应用研究

摘要

In acid medium, the interaction between fsDNA and Eriochrome Blue Black R （EBBR） in pretence of cetyltrimethylammonium bromide （CTMAB） Was studies using RLS method respectively. RLS spectra of the system Was measured and the influence factors were investigated. Under optimized condition, there is a linear relationship between the concentration of fsDNA and RLS intensity of the system （EBBR- CTMAB -fsDNA） in the ranges from 0.01 to 1.6 μg/mL, with detection limit of 4.6 ng/mL （3σ,n = 5）, respectively. This new approach was applied to determine trace nucleic acids in synthetical sample, the recovery vercent was 97% to 106% and RSD was less than 2.8%.%本文用RLS技术研究了在酸性介质中，阳离子表面活性剂溴化十六烷基三甲铵（CTMAB）存在下，依来铬蓝黑R（Eriochrome Blue Black R，EBBR）与鱼精脱氧核糖核酸（fsDNA）相互作用的RLS光谱特征及影响因素，并在优化实验条件下，实现了对fsDNA的定量检测。对于EBBR┝CTMAB—fsDNA体系，fsDNA浓度的线性范围为0．01-1．6μg／mL，相应的检出限（3σ，n=5）为4．6ng／mL。将所建立的方法用于合成样品中痕量核酸的测定，回收率在97％-106％之间，RSD小于2．8％。