功能性L-Cys-CdS纳米粒子共振光散射法测定脱氧核糖核酸

摘要

Aim To determine nucleic acid (DNA) using Nanometer-sized L-cysteine-capped CdS particles by resonance light scattering (RLS) method. Methods The nano-particles synthesized by a colloidal aqueous method were water-soluble, stable, and highly luminescent. The RLS of L-Cys-CdS particles were greatly quenched by DNA in Tris-HCl solutions. The intensity of RLS at 344 nm was pro portional to the concentration of DNA. Results The linearity range of the calibration curve was 0. 01 - 1.0 μg· mL- 1 for calf thymus DNA and 0. 04 - 1.5 μg· mL-1 for salmon sperm DNA, The detection limits (3 δ) were 8 ng·mL-1 for calf thymus DNA and 10 ng·mL-1 for salmon sperm DNA. Conclusion This method is simple, sensitive, and capable of avoiding the use of toxic dyes.%目的报道了功能性L-Cys-CdS纳米荧光探针的合成,纳米粒子水溶性好,稳定,且具有较好的荧光特性.方法以该纳米粒子为荧光探针,探讨了DNA对其的荧光猝灭作用.结果表明在Tris-HCL溶液中DNA对CdS的RLS有很好的猝灭,小牛胸腺DNA及鱼精DNA的线性范围及检测限分别为0.01-1.0μg·mL-1,8 ng·mL-1;0.04-1.5μg·mL-1,10ng·mL-1.结论该方法简便、快速、灵敏,用于测定合成样品取得了满意的结果.