光谱法研究安乃近与牛血清白蛋白的相互作用及共存金属离子的影响

摘要

【目的】旨在研究安乃近（ANG）与牛血清白蛋白（BSA）的相互作用，为安乃近的临床研究提供一定的参考依据。【方法】在优化的实验条件下，运用光谱法研究牛血清白蛋白（BSA）与安乃近（ANG）的相互作用及共存金属离子的影响。【结果】研究表明：ANG 对 BSA 的荧光有猝灭作用，属于静态猝灭。BSA 能运输 ANG，是一个自发过程，其作用力类型主要为氢键或范德华力。BSA 的亚螺旋域ⅡA 是主要结合位置，离酪氨酸残基更近，有微弱的药物负协同作用。ANG 对 BSA 构象产生影响。Cr3＋、Co2＋、Fe3＋、Mn2＋和 Mg2＋对 ANG 与 BSA 结合有竞争作用，增强药效。【结论】ANG 确实与 BSA 发生了相互作用，金属离子对两者结合产生影响。%Objective]The interaction of Analgin (ANG)with bovine serum albumin (BSA)was investigated so as to provide references for its clinical application.[Method]Under the optimal conditions,the interaction of ANG with BSA and the effect of coxisting metal ions were investiga-ted by spectrometry.[Results]The results showed that the fluorescence of BSA was quenched by ANG.It was a static quenching process.BSA could transport ANG,which was spontaneous and mainly driven by Der Waals forces or hydrogen bond.The primary binding site for ANG was loca-ted at sub-domain ⅡA of BSA ,which was near by tyrosine residue.There was some negative co-operative effect.The conjugation reaction could affect the conformation of BSA,which led to the polarity around BSA strengthened.Mn2 + ,Fe3 + ,Cr3 + ,Zn2 + and Mg2 + competed with the inter-action,which could increasing medical effectiveness.[Conclusion]ANG can interact with BSA and the interaction was affected by metal ions.