莫娜百合组培快繁灭菌和诱导条件的优化

摘要

对观赏百合品种莫娜鳞片进行组培快繁条件的优化研究．结果表明，使用75％酒精消毒10s，0．1％升汞处理10min，最后用无菌水清洗5次灭菌效果最好，污染率最低；MS＋0．5mg／L6-BA＋1mg／LNAA为最佳诱导培养基．%Optimization of sterilization and induction conditional for tissue culture and rapid propagation of Lilium Mona were studied. The results show that when sterilized for 10s by 75% alcohol, treated for 10min by 0.1% mercuric chloride, and then cleaned 5 times by sterile water, the lowest contamination rate can be achieved. It is also pointed out that the best induction medium is MS＋0.5 mg/L 6-BA＋ 1 mg/L NAA.