In 200 mmol/L phosphate buffer with pH6. 0, the reaction of N , N:ethylenebis [2- ( o-hydroxyphenolic)glycine] with cupric ion was monitored by UV difference and fluorescence spectra. The molar absorptivity of Cu(Ⅱ)-EHPG at 265 nm is (5. 462 ± 0. 035) ×104 cm-1 · mol-1 · L. The conditional binding constant is logKCu-EHPG =15. 62±0. 18.%在pH 6.0,200 mmol/L磷酸盐缓冲溶液中使用紫外差光谱、荧光光谱研究了Cu(Ⅱ)与N,N'-乙烯-二[2-(2-羟基苯基)苷氨酸](EHPG)的结合.结果表明Cu(Ⅱ)可与EHPG形成1∶1的稳定配合物,其在265 nm处的摩尔吸光度为(5.462±0.035)× 104 cm-1·mol-1·L.配合物条件结合常数为:logKCu-EHPG=15.62±0.18.
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