前交叉韧带重建早期移植肌腱的生物力学和关节滑液中IL-1β的变化

摘要

Objective To establish the model of anterior cruciate ligament reconstruction in rabbits,and explore the relationship between the expression of interleukin 1β in synovial fluid and the biomechanical changes of transplanted tendon in the early stage after operation.Methods Twenty-five healthy adult New Zealand rabbits were selected,with one side of hind legs as experimental group and the contralateral as normal control group.The anterior cruciate ligament reconstruction model was reconstructed with the ipsilateral hallux longus tendon.All animals were executed at 2,4,6 week after surgery.The biomechanical changes of transplanted tendon was determined.The expression level of IL-1β in synovial fluid was detected by ELISA.Results ELISA results showed that the level of IL-1β in experimental group peaked at 2 week after surgery,and then decreased gradually to the normal level at the end of 6 weeks.At 2,4 and 6 week after surgery,the tensile force of ligament rupture in experimental group was significantly lower than that in normal control group(P ＜0.01).The strength of the reconstructed ligament decreased rapidly and reached the lowest at the end of 4 weeks.Conclusion The level of IL-1β in synovial fluid has an adverse effect on the biomechanical strength of grafts after anterior cruciate ligament reconstruction.The results suggest that the decrease of IL-1β level in the joint is one of the positive factors to improve the biomechanical strength of the transplanted tendon.%目的 建立兔前交叉韧带(anterior cruciate ligament,ACL)重建模型,探讨术后早期关节滑液中白细胞介素1β的表达水平及移植肌腱生物力学变化的关系. 方法 25只成年新西兰大白兔,一侧后肢作为实验组,使用同侧踇长伸肌腱建立前交叉韧带重建模型,对侧作为正常对照组,于术后2,4,6周分别处死,测定移植肌腱的生物力学变化,并用ELISA法检测关节滑液中白细胞介素1β的表达水平. 结果 ELISA测定结果显示,实验组IL-1β水平在术后第2周达到高峰后逐渐下降,第6周接近正常水平.术后2,4,6周实验组韧带断裂所需拉力明显低于正常对照组(P＜0.01),术后重建韧带强度急剧下降,4周末时达到最低,后强度逐渐升高. 结论 前交叉韧带重建手术后关节滑液中IL-1β的水平变化对移植物生物力学强度产生不利影响,提示降低关节内IL-1β水平是改善移植肌腱生物力学强度的积极因素之一.