碱性成纤维细胞生长因子对大鼠急性脊髓损伤后内质网应激蛋白Caspasel2表达的影响

摘要

目的 观察碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对大鼠急性脊髓损伤后内质网应激蛋白Caspasel2表达的影响. 方法 90只8-10周SD大鼠随机分为假手术组(sham)、急性脊髓损伤(SCI)组和bFGF治疗组(SCI+ bFGF),分别于损伤后12h,ld,3d,7d和14 d,采用Rivlin斜板试验评估大鼠运动功能;采用TUNEL法检测神经细胞凋亡,免疫组化法观察受损节段脊髓组织中Caspasel2的表达. 结果 SCI组和SCI+ bFGF组各个时间点斜板度数均值与sham组比较,差异有统计学意义(P＜0.01);SCI+ bFGF组Rivlin斜板试验角度7 d(36.30°±2.82°)、14 d(46.50 ±2.98)与SCI组7 d(28.20°±2.78°)、14 d(33.30°±2.88°)比较,差异有统计学意义(P ＜0.05,0.0l);SCI组较sham组细胞凋亡增加[(14.20-+2.12)％vs(2.35±0.62)％,P＜0.01],SCI+ bFGF组[-(10.32±1.68)％]较SCI组细胞凋亡减少(P＜0.01);Caspasel2的表达SCI组较sham组升高(12.20±1.02 vs2.10 ±0.20,P＜0.01),SCI+ bFGF组(7.50 ±0.60)较SCI组降低(P＜0.01). 结论 急性脊髓损伤后Caspasel2的表达明显升高,bFGF对Caspasel2的表达具有抑制作用.%Objective To explore the effect of basic fibroblast growth factor(bFGF) on the expression of Caspase12 after acute spinal cord injury in rats.Methods Ninety rats were randomly divided into sham group,acute spinal cord injury (SCI) group and bFGF group (SCI + bFGF)at 8-10 week.At 12 h,1 d,3 d,7 d and 14 d after acute spinal cord injury,the movement function was evaluated in rats by Rivlin inclined plate experiment,Neural cell apoptosis was examined by TUNEL staining and the expression of Caspase12 was detected in the damaged segments of the spinal cord by immunohistochemical staining.Results Rivlin inclined plate experiment average angle showed significantly difference between SCI group,SCI + bFGF group and sham group at each time point(P ＜ 0.01).Rivlin inclined plate experiment average angle was significantly higher in SCI + bFGF group than in SCI group at 7 d(36.30° ±+ 2.82° vs 28.20° ±+ 2.78°,P ＜ 0.05) and 14 d(46.50° ±+ 2.98° vs 33.30° ±+ 2.88°,P ＜ 0.01).The cell apoptosis was significantly higher in SCI group than in sham group(14.20 +2.12 vs 2.35 +-0.62,P ＜0.01),and it was lower in SCI + bFGF group(10.32 + 1.68) than in SCI group(P ＜ 0.01).The expression of Caspase12 was significantly higher in SCI group than in sham group(12.20 ± 1.02 vs 2.10 ±+0.20,P＜0.01),and it was lower in SCI +bFGF group(7.50 ±0.60)than that in SCI group(P＜0.01).Conclusion The ex pression of Caspase12 increases after acute spinal cord injury in rats,and bFGF can inhibit the expression of Caspase12.