雌激素对去卵巢大鼠焦虑行为及杏仁核c-fos表达的影响

摘要

目的 观察雌二醇对去卵巢大鼠焦虑样行为及杏仁核c-fos表达的影响,探讨雌二醇改善去卵巢大鼠焦虑样行为的可能机制.方法 30只SD雌性大鼠随机分为假手术组、卵巢切除组和去卵巢雌二醇组.雌二醇治疗8周后通过高架迷宫实验观察雌二醇对去卵巢大鼠焦虑样行为的影响;随后采用免疫组织化学结合图像半定量方法对杏仁核c-fos的表达进行测量和分析.结果 与假手术组大鼠比较,高架迷宫实验中卵巢切除组大鼠开放臂次数及时间、开放臂次数比例及时间比例均显著下降(P＜0.01),杏仁核c-fos数量及面积均显著增加(P＜0.05);而去卵巢雌二醇组去卵巢大鼠的焦虑行为可显著改善,杏仁核c-fos表达减弱(P＜0.05).结论 卵巢摘除会导致大鼠焦虑样行为,长期雌二醇治疗可改善去卵巢大鼠的焦虑行为,可能与雌二醇降低去卵巢大鼠杏仁核c-fos的表达有关.%Objective To explore the influence of estradiol on anxiety-like behavior and amygdala c-fos expression and the possible mechanism of estradiol improving anxiety-like behavior of the ovariectomized(OVX) rats.Methods Thirty adult SD female rats were randomly assigned into 3 groups:sham group,OVX group and OVX + E2 group(ovariectomized rats treated with estradiol).The elevated plus maze was used to assess the anxiety-like behavior after 8-week estradiol treatment.Immunohistochemical staining was used to detect the c-fos expression in amygdala.Results Compared with sham group,open ann entry(OE),open arm time(OT),the percentage of OE and the percentage of OT significantly decreased in OVX group(P ＜0.01),while the number and area of amygdala c-fos expression significantly increased(P ＜ 0.05).Compared with OVX group,anxiety-like behavior was improved and amygdala c-fos expression decreased after 8-week estradiol treatment in OVX + E2 group(P ＜ 0.05).Conclusion The ovariectomy may induce the anxiety-like behavior,and estradiol may ameliorate the anxiety-like behavior in OVX rats by decreasing the amygdala c-fos expression.