过表达CD82基因对口腔鳞癌OSCC-25细胞增殖、侵袭和凋亡的影响

摘要

目的 探讨CD82在口腔鳞癌OSCC-25细胞的表达情况及过表达CD82基因对口腔鳞癌细胞增殖和侵袭、细胞凋亡的影响. 方法 将真核表达载体pIRES2-EGFP-CD82转染至口腔鳞癌OSCC-25细胞中,分别培养0,12,24,48,72 h,并在各个时点采用Western blot观察CD82蛋白表达情况并确定表达效率最高时间.同时以未转染的OSCC-25细胞为空白对照组,OS-CC-25细胞加入空白质粒为阴性对照组,实验分别通过MTT,Transwell小室及Annexin Ⅴ-FITC/PI双染细胞凋亡检测方法检测其对OSCC-25细胞增殖、侵袭及细胞凋亡的影响. 结果 转染pIRES2-EGFP-CD82真核表达质粒载体72 h,Western blot结果显示CD82蛋白水平明显增高(P<0.05).与空白及阴性组相比较,实验组OSCC-25细胞的增殖、侵袭能力均受抑制,细胞凋亡水平上升,且差异具有统计学意义(P<0.05). 结论 在体外活细胞实验中,过表达CD82基因可有效抑制口腔鳞癌OSCC-25细胞的增殖和侵袭能力,并促进鳞癌细胞的凋亡.%Objective To investigate the protein levels of CD82 and explore the effects of its overexpression on OSCC-25 cell prolifera-tion,invasion and apoptosis. Methods The eukaryotic expression vector pIRES2-EGFP-CD82 was transfected into OSCC-25 cells. The protein levels of CD82 were examined using Western blot at 0 h,24 h,48 h and 72 h after transfection. The non-transfected cells were chosen as blank group and the cells transfected with the NC plasmid group were chosen as negative control group. Cell prolifera-tion,invasion and apoptosis were assessed by MTT,Transwell and Annexin Ⅴ-FITC/PI staining,respectively. Results When the eukaryotic plasmid pIRES2-EGFP-CD82 was transfected at 72 h,the expression of CD82 protein was highly increased using Western blot analysis(P<0.05).Compared to blank group and negative group,the proliferation and invasion of OSCC-25 cells were significant-ly inhibited in CD82 transfection group,and the apoptotic rate of OSCC-25 cells was increased(all P<0.05). Conclusion The overexpression of CD82 may inhibit the proliferation and invasion of OSCC-25 cells,but enhance the apoptotic of OSCC-25 cells in vitro.