一种改良的真菌总RNA提取方法

摘要

以棉花黄萎病菌株VD8为试材,利用改良的CTAB提取RNA方法,应用LiC[选择性沉淀RNA,减少沉淀时间,在醋酸钠条件下用异丙醇祛除多糖,结果表明,利用该方法从富含多糖的黄萎病菌菌丝中获得了质量较好的总RNA,缩短了提取时间.%With Verticillium dahliae VD8 fugus material, LiCl to selective deposit RNA, in shortened deposition time, under the condition of sodium acetate, using Isopropyl alcohol to clear polymeric, carbohydrates , an improved CTAB method was developed for extracting total RNA from Verticillium dahliae Vda fungus that are rich in polymeric carbohydrates, and extracting time shortened.