4种PCR体系对湖北海棠DNA扩增效果的对比

摘要

The experiment with four PCR systems (2 × Taq MasterMix,Forward Primer,Reverse Primer,Template DNA,RNase-free Water;,2 × PCR buffer,2 mmol/L dNTPs,Forward Primer,Reverse Primer,KOD FX,Template DNA,RNase-free Water;10 × Buffer for Blend Taq,Blend Taq,Template,Forward Primer,Reverse Primer,dNTPs,RNase-free Water; 10 × Taq PCR Buffer,dNTP Mix,Forward Primer,Reverse Primer,MgCl2,Template DNA,Taq DNA Polymerase,RNase-Free Water),were used respectively to test the different content and quality of Malus hupehensis (Pamp.)Rehd for the PCR experiments.The results showed:the DNA diluted for several times had little influence on the four PCR systems and it could amplilied a clear,bright band,but different quality DNA had a great effect on these four systems.The bad quality DNA could only amplilied a clear,bright band in the mix system and other three systems failed.%用4种PCR体系(2×Taq MasterMix,Forward Primer,Reverse Primer,Template DNA,RNase-free Water；2×PCR buffer,2 mmol/L d NTPs,Forward Primer,Reverse Primer,KOD FX,Template DNA,RNase-free Water; 10×Buffer for Blend Taq,Blend Taq,Template,Forward Primer,Reverse Primer,dNTPs,RNase-free Water; 10×Taq PCR Buffer,dNTP Mix,Forward Primer,Reverse Primer,MgCl2,Template DNA,Taq DNA Polymerase,RNase-free Water),分别对不同含量和质量的湖北海棠DNA进行PCR实验.结果表明,DNA稀释几倍后对4种PCR体系的影响不大,扩增的条带清晰;但不同质量DNA对4种PCR体系影响很大,低质量DNA只能在混合酶体系下才可以扩增出清晰、明亮的条带,而其他3个体系下的扩增产物均不清晰.