首页> 外国专利> PCR PRIMER SET FOR BACTERIAL DNA AMPLIFICATION, KIT FOR DETECTING AND/OR IDENTIFYING BACTERIAL SPECIES, AND METHOD FOR DETECTING AND/OR IDENTIFYING BACTERIAL SPECIES

PCR PRIMER SET FOR BACTERIAL DNA AMPLIFICATION, KIT FOR DETECTING AND/OR IDENTIFYING BACTERIAL SPECIES, AND METHOD FOR DETECTING AND/OR IDENTIFYING BACTERIAL SPECIES

机译:用于细菌DNA扩增的PCR引物,试剂盒检测和/或鉴定细菌物种,以及用于检测和/或鉴定细菌种类的方法

摘要

An object of the present invention is to provide a primer set useful for further improvement of sensitivity in detection or identification of bacterial species in a sample by a PCR method, a kit for PCR using the primer set and a method of detection or identification of bacterial species in a sample using the primer set, as well as to further improve sensitivity in detection or identification of bacterial species in a sample by conducting a PCR method using the primer set with a minimized contamination amount of bacterial nucleic acid. A primer set according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups S1 to S7 consisting of specific primers in order to achieve the above objects. In addition, a kit for detection of bacterial species according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups K1 to K7 consisting of specific primers.
机译:本发明的一个目的是提供一种有用的引物,其可用于通过PCR方法进一步改善样品中的细菌种类的细菌种类的敏感性,使用引物组的PCR试剂盒和一种检测或鉴定细菌的方法使用引物组的样品中的种类,以及通过使用具有最小化细菌核酸的底漆进行PCR方法,进一步提高样品中的细菌种类的检测或鉴定的灵敏度。根据本发明的引物组在通过从组S1至S7中的每一个由特定引物组成以实现上述目的而获得的七个引物对中的至少一种引物对。另外,用于检测根据本发明的细菌物质的试剂盒在通过从组K1至K7中的每一个组成的组组成的组组成的七个引物对中的至少一个引物对。

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