拟南芥35S∶MS606/myb26转基因植物的鉴定

摘要

ms606是山西大学植物生殖发育实验室创制的雄性不育突变体,该突变体具有药室内壁次生加厚缺陷,导致花药不能开裂.而转录因子MYB26在药室内壁次生加厚过程中发挥着重要的调控作用.为了研究MS606和MYB26之间的关系,我们将35S∶MS606融合基因在农杆菌介导下转化植物myb26/MYB26杂合体,通过潮霉素抗性筛选,结果获得T1转化植株;并通过在基因组水平的PCR检测和RNA水平的半定量RT-PCR检测,获得纯合的35S∶MS606/myb 26转基因植株.该材料的获得为深入研究MS606和MYB26基因在调控药室内壁次生加厚途径中的关系奠定了良好基础.%ms606 is a male sterile mutant with defect in anther dehiscence.Anthers from the ms606 plants fail to dehiscence due to loss of endothecium secondary thickening.The transcription factor MYB26 plays a regulatory role in endothecium lignification.Therefore,the relationship between MS606 and MYB26 is deserved to investigate.The 35S:MS606 cDNA construct was introduced into the myb26 background to determine whether MS606 overexpression had any effect on myb26 mutant phenotype.The transgenic plants were selected by hygromycin resistance and confirmed by PCR genotyping.RT-PCR showed that MS606 was overexpressed in homozygous 35S:MS606/myb26 transgenic plants.This study has provided a basis for further investigating the relationship between MS606 and MYB26 on endothecium secondary thickening in Arabidopsis.