江西铅山红芽芋试管苗染色制片技术优化研究

摘要

以江西铅山红芽芋试管苗为材料，探讨不同取材部位、不同预处理剂、不同酸解离时间和不同改良卡宝品红染色时间对江西铅山红芽芋试管苗染色体制片的影响，以期为江西铅山红芽芋的起源、演化及遗传育种提供一定理论依据。结果表明，切取江西铅山红芽芋试管苗根尖，在室温下用0．1％秋水仙素＋0．002mol／L 8－羟基喹啉混合溶液预处理4h ，然后在60℃条件下用1 mol／L盐酸解离15 min ，最后用改良卡宝品红染色5 min ，此时染色体制片效果最佳。%In order to better understand the information about cytology of Jiangxi Yanshan red bud taro ( Colocasia esculenta L .Schott var .cormosus cv .Hongyayu) ,provide information support for genetic breeding of red bud taro .The protocol of different sampling places ,pretreatments solutions ,dissociating time and dying time on chromosome observation from red bud taro plantlets was studied . The results showed that the effect of chromosome preparation was best when the root tips of red bud taro plantlets was cut ,pretreated in a mixture of 0 .1% colchicines and 0 .002mol/L 8-hydroxyquinoline at 25 ℃ for 4h ,dissocated in 1 mol/L HCl at 60 ℃ for 15 min and dyed with modified carbol fuchsin for 5 min .