Q uEChERS －毛细管电泳法测定紫薯中的农药残留

摘要

将QuEChERS与毛细管胶束电动色谱法相结合，建立了一种快速、高效检测紫薯中三嗪类农药残留含量的方法．在QuEChERS处理样品过程中，乙腈作为萃取剂，伯仲胺和石墨化碳黑作为样品净化的吸附剂．毛细管电泳的分离缓冲液为30 mmol／L Tris－HCl－40 mmol／L SDS－10％甲醇（V／V），pH＝7．77，分离电压15 kV，进样时间7 s．在最佳条件下，嗪草酮、环嗪酮和氰草津的检出限分别为60 ng／mL、60 ng／mL、20 ng／mL，实际样品检测的加标回收率在82．1％～110．0％之间．%A rapid and highly efficient method was developed to determine triazine herbicides in purple potato combined with QuEChERS and micellar electrokinetic capillary chromatography ( MEKC ) .In the process of QuEChERS, acetonitrile was used as extraction agent, and primary secondary amine ( PSA) and graphitized carbon black ( GCB) were used as sample purification adsorbent.The optimum separation conditions are running buffer 30 mmol/L Tris-HCl-40 mmol/L SDS-10% methanol(V/V), pH 7.77, 15 kV of separation voltage, 7 s of injection time and DMSO as the electroosmosis marker.The detection limits of three model herbicides, metribuzin, hexazinoe and cyanazine are 60 ng/mL, 60 ng/mL and 20 ng/mL, respectively.The recovery rate was between 82.2%and 110.0%.