The influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes

摘要

Objective: To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse's oocytes.Methods: A total of 15 KunMing mice aged 10-14 weeks were undergone ovulation induction and oocytes collection.The oocytes were randomly divided into three groups.The first group was the control group and cultured without macrophage; the second group (experimental group 1) was co-cultured with macrophages right after oocytes collection; the third group (experimental group 2) was cultured alone after oocytes retrieval, and co-cultured with macrophages from 8 cell stage.Results: 1.The fertility rate in control group (91.0%) and experimental group 2 (93.7%) was significantly higher than that in experimental group 1 (72.0%, P＜0.001).2.Blastocyst formation rate of the fertilized oocytes in the experimental group 2 (78.2%) was much higher than control group (60.3%, P＜0.001).3.Blastocyst formation rate of the oocytes in experimental group 2 (73.2%) was much higher than control group (54.8%) and experimental group 1 (50.0%), which were statistically significant.Conclusions: 1.Co-culture with peritoneal macrophages had an adverse influence on the fertilization process.2.Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.