Objective:To evaluate the influencing factors of single blastocyst transfer in frozen-thawed cycles.Methods:A total of 109 cycles with single blastocyst transfer after thawing were analyzed in the First Affiliated Hospital of Sun Yat-sen University from January 2011 to August 2012.Among these cycles,48 blastocysts were frozen on D5 after insemination (D5 group) and 61 were frozen on D6 (D6 group).Results:Implantation rate in D5 group was significantly higher than D6 group (45.83％ vs.24.59％) (P＜0.05).The abortion rate in D5 group was higher than D6 group (9.09％ vs.26.67％),but no statistical difference was found (P ＞ 0.05).The logistic regression analysis showed that neither blastocysts developmental stage,nor morphology of inner cell mass or trophectoderm was significantly related to implantation potential (P ＞ 0.05,95％ CI 0.879-3.061,0.318-3.184,0.328-1.362 respectively).However,blastocysts developmental stage on D6 was significantly related to implantation potential(P＜0.05,95％ CI 1.080-10.013).Conclusion:Clinical outcomes of D5 single blastocyst transferred cycles were better than D6 blastocysts.D6 blastocysts expansion may be the most important parameter when a single blastocyst is selected for transfer.%目的 分析玻璃化冻融后单个囊胚移植周期中妊娠结局的影响因素. 方法 回顾性分析中山大学附属第一医院2011年1月至2012年8月的109个单个冻融囊胚移植周期资料.根据囊胚玻璃化冷冻时的受精天数,将其分为2组:D5单囊胚移植组(n=48)、D6单囊胚移植组(n=61).比较两组的胚胎复苏率、植入率和流产率,并分析囊胚形态参数预测胚胎植入潜能的价值. 结果 D5单囊胚移植组的植入率为45.83％,D6单囊胚移植组为24.59％,两组比较有统计学差异(P＜0.05).D5移植组的流产率有低于D6移植组的趋势(9.09％ vs.26.67％),但差异无统计学意义(P＞0.05).Logistics回归分析结果显示,囊胚分期、内细胞团和滋养外胚层形态均与植入潜能无显著相关性(P＞0.05,95％CI分别为0.879～3.061、0.318～3.184和0.328～1.362).然而分别对D5与D6囊胚进行分析,结果显示D6囊胚扩张程度与植入潜能相关(P＜0.05,95％CI 1.080～10.013). 结论 D5单囊胚移植结局优于D6单囊胚移植；D6囊胚的发育速度与囊胚的植入潜能相关.
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