孕激素受体基因单核苷酸多态性与子宫内膜胞饮突表达延迟的相关性研究

摘要

Objective:To clarify the potential relationship between single nucleotide polymorphisms (SNP)in progesterone receptor (PGR) gene and retardation expression of pinopode.Methods:Forty patients with recurrent implantation failure(RIF)who were diagnosed as retardation expression of pinopode by scanning electron microscopy examination at window period were recruited in the reproductive medicine center of Xiangya Hospital from August 2015 to December 2015.At the same period,40 natural conception and term pregnant women were recruited as control from Yiyang Special Hospital Affiliated to Yiyang Medical College.5 ml peripheral blood was collected from every participant for the test of single nucleotide polymorphism(PCR-RFLP)of exon receptor.The results of the two groups were compared.Results:Eighty blood samples were successfully tested for progesterone receptor gene base pairing sequencing.Among 80 women,the overall SNP incidence was 42.50％ (34/80).The single nucleotide polymorphisms were detected in 18 sample of the observation group and 16 of the control group.The SNP incidence was not significantly different between the two groups(45.00％ vs.40.00％,P＞0.05).Conclusions:The single nucleotide polymorphism of the progesterone receptor gene is not the main cause of delay in opening planting window.%目的 探讨孕激素受体的基因单核苷酸多态性(SNP)与不孕症患者子宫内膜窗口期开启延后的相关性.方法 招募2015年8～12月在中南大学湘雅医院生殖中心反复移植未孕且经窗口期扫描电镜检查诊断胞饮突表达延后的原发不孕患者40名为观察组,另招募同期益阳医专附属医院自然受孕并足月待产妇40名为对照组.取外周肘静脉血5 ml,进行孕激素受体基因8个外显子SNP位点测序,比较两组的检测结果. 结果 观察组患者内膜样本的扫描电镜检查提示该组患者内膜种植窗的开启较理论时间延后.受检的80例血标本均成功进行了孕激素受体基因碱基对序列检测,其中有34人检测到SNP,总发生率为42.50％(34/80);观察组患者中18位(45.00％)检测到SNP,对照组中16位(40.00％)检测到SNP,两组SNP发生频率无显著性差异(P＞0.05). 结论 孕激素受体基因SNP不是导致种植窗开启延迟的主要原因.