Objective To evaluate the sensitivity and specificity of anti-cyclic citrullinated peptide antibody ( anti-CCP ) in patients with rheumatoid arthritis ( RA ). Methods Anti-CCP antibedies were examined by using MEIA and ELISA from 69 RA, 30 Ankylosingspendylitis ( AS ), 33 Siccasyndrome ( SS ) and 61 normal donors sera. Results Among 69 petients with RA, The sensitivity and specificity ef anti-CCP were ( 69.56%, 68.11% ), ( 96.77%, 97.26% ) by MEIA and ELISA in RA respeedvely. The data showed that there was a significant difference between periods of activity and periods of quiescence, However, no difference was found between the results in RA by using MEIA and ELISA. Conclusion Detecting anti-CCP by MEIA may be a brief, standard and reliable method. It might present more sensitive and specific in clinical diagnosis on RA.%目的:应用化学发光法、酶联免疫吸附试验(ELISA)定量检测类风湿性关节炎(RA)患者抗环瓜氨酸多肽(CCP)抗体的含量,探讨CCP抗体在RA早期诊断和治疗中的作用.方法:选取69例RA、30例强直性脊柱炎(AS)、33例干燥综合征(SS)患者、61例健康体检人群,分别用化学发光、ELISA检测其血清CCP抗体的含量.结果:两种方法检测193例样本CCP抗体,阳性结果符合率为98.08%,阴性符合率为100%,总符合率为99.48%,CCP抗体对RA诊断的敏感性、特异性分别为(69.56%,68.11%)、(96.77%,97.26%)、RA患者活动期CCP抗体含量(141.10±93.95)IU/ml与非活动期(45.14±10.54)相比,有显著性差异(P<0.05).结论:化学发光、ELISA两种方法定量检测CCP抗体的符合率良好,化学发光法适用于仪器自动化,操作简便,易于标准化,可满足临床的需要.CCP抗体是一种新的对RA有较高诊断价值的血清学标志物,有助于对RA病情监测.
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