Objective To study the distribution of hepatitis C genotypes in Changzhou region,and the relationship between the viral replication and liver dysfunction. Methods Hepatitis G virus antibody (HCV-Ab) positive serum samples of 150 patients were collected and serum hepatitis G virus RNA(HCV RNA) level was detected by using FQ-PCR and alanine aminotransferase(ALT) , aspartate amin-otransferase( AST) were also detected. Besides, HGV was genotyped by gene chip. Results 150 cases were divided into two groups in-cluding HCV RNA negative group(1×103/ml). Serum ALT and AST levels in the positive group were higher than those in the negative group (P0.05). Conclusion Hepatitis G virus in Changzhou region is mainly type 1 virus, and sustained HCV replication may cause liver dysfunction and it is important to detect HCV RNA,ALT and AST in clinical treatment.%目的:了解常州地区丙型肝炎病毒基因型分布、病毒复制与肝功能损害的关系.方法:对150例丙型肝炎病毒抗体检测为阳性的患者,采用荧光定量聚合酶链反应技术检测血清丙型肝炎病毒核糖核酸(HCV RNA)水平,并检测丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)等肝功能相关指标,HCV基因分型采用基因芯片法.结果:150例患者中,HCV RNA阳性患者130例(>1×103IU/ml),HCV RNA阴性患者20例(0.05),HCV RNA阳性患者血清ALT和AST指标明显高于HCV RNA阴性患者(P0.05).结论:常州地区丙型肝炎病毒感染以1型病毒为主;持续的丙型肝炎病毒复制与肝功能损害密切相关;病毒载量与肝功能检测对丙型肝炎的治疗有重要意义.
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