安多霖对高功率微波辐照大鼠睾丸生精细胞rCaspase-9和XIAP的影响

摘要

为探讨安多霖对微波辐射损伤的的防治机制,将SD雄性大鼠按质量随机分为正常对照组、辐照组和预防组(给药量分别为3、6、9 mg/kg).预防组大鼠连续灌胃给药,对照组和辐照组给予双蒸水,20 d后辐照组和预防组大鼠行100 mW/cm2的高功率微波(High power-microwave,HPM)全身辐照10 min.于辐照后第1、2、5天取睾丸组织制备病理标本,采用SP免疫组化检测试剂盒标定目的蛋白,Image-pro plus图像分析软件对特定染色区域进行灰度分析和测定.结果显示,辐照组大鼠睾丸细胞Caspase-9水平较对照组呈"高?低?高"的"倒抛物线"形(p<0.05),给药组则随着时间呈现"先低后高"的趋势(p<0.05);对于X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)而言,辐照组与对照组之间的差异不显著,而6、9 mg/kg组在辐照后第5天较辐照组显著升高(p<0.05).结果提示,Caspase-9参与了HPM致大鼠睾丸细胞凋亡的过程,安多霖对大鼠睾丸细胞的保护作用需要Caspase-9和XIAP的共同参与.%In order to investigate the Anduolin injury prevention mechanism of microwave exposure, sexually matured SD rats were randomly divided into control group, exposure group, and prevention groups (drug dosage were 3, 6 and 9 mg/kg). Prevention groups were given drug continuously by oral administration for 20 d, meanwhile the control group and exposure group were given distilled water. After that the prevention groups and exposure group were exposed to high power-microwave(HPM) of 100 mW/cm2 for 10 min. Rats were killed at 1st, 2nd, and 5th day after exposure, and the pathological specimens were prepared from testicular tissue. Interested proteins in testicular tissue were detected with immunohistochemical method, and the protein content were analyzed with image-pro plus analysis software. The results showed that protein content of Caspase-9 of exposure group showed a high-low-high trend compared with control group (p<0.05) at 1st, 2nd, and 5th day after exposed to HPM, while the prevention groupsshowed a trend from low to high with time (p<0.05); for X-linked inhibitor of apoptosis protein (XIAP) protein, there was no significantly difference between exposure group and control group, while the XIAP levels of 6 mg/kg group and 9 mg/kg group at 5th day after exposure increased significantly compared with the exposure group (p<0.05). The results suggest that Caspase-9 is involved in the HPM-induced apoptosis in rat testis, and protective effect of Anduolin on rat testicular cells requires participation of Caspase-9 and XIAP.