下颌骨骨折愈合过程中整合素β1表达的免疫组化研究

摘要

目的：探讨下颌骨骨折愈合过程中整合素β1表达水平的改变。方法：用兔作下颌骨骨折的动物模型，分别于骨折前一天及骨折后第1、3、5、7、14、30、60、90 天取骨断端组织标本作成石蜡切片并用LsAB法进行免疫组织化学实验，以探讨整合素β1在骨组织，特别是在成骨细胞和破骨细胞中的表达。结果：整合素β1在骨组织及其周围软组织中广泛表达；成骨细胞上的整合素β1在7 d后表达增强，14～30 d最为强烈，90 d时接近正常。破骨细胞上整合素β1在骨折后14 d表达增强，一直延续到90 d。结论：在下颌骨骨折的愈合过程中，整合素β1在骨组织，特别是在成骨细胞和破骨细胞上的表达增强，整合素β1对骨折的愈合过程具有影响作用。%Objective:To investigate the expression of integrin β1 during mandibular fracture healing.Methods:Rabbit mandibular fracture model was used,the fractured bone tissues were obtained and paraffin slices were made on the day 1,3,5,7,14,30,60 and 90 after surgery respectively.Non-fractured mandible was used as the control.The expression of integrin β1 in bone tissue, especially in osteoblasts and osteoclasts, was studied with LsAB immunohistochemical method.Results:Integrin β1 was widely espressed in the bone tissure close to fracture and surrounding soft tissue.In osteoblasts increased expression of integrin β1 was observed on day 7,maximum on day 14～30 and normal on day 90.While in osteoclasts,it was increased and kept at maximum 14～90 days aftere fracture.Conclusion:During mandibular fracture healing,the expression of integrin β1 in bone,especially in osteoblasts and osteoclasts,was increased.Integrin β1 has a critical influence on bone fracture healing.