Objective: To identify the different protein expression profiles between human oral squamous cell carcinoma (OSCC) and normal oral mucosa tissues, and provide experimental data for further study of the development mechanism of OSCC. Methods: 10 cases of OSCC and paired normal oral mucosa tissues were collected and analyzed through two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Results: (1) The average protein spots of OSCC were 2 325±390, while that of normal oral mucosa tissues were 2 487±281. (2) 29 differential protein spots were found between OSCC and normal oral mucosa. Moreover, these protein spots were all down- regulated in OSCC compared with normal oral mucosa. Among these spots, 3 were identified as fibrin beta, triosephosphate isomerase (TIM) and unknown protein through mass spectrometry and bioinformation. Conclusion: Down-regulation of fibrin beta, Triosephosphate isomerase(TIM) and unknown protein are found in the development of OSCC and the mechanism needs further study.%目的:筛选口腔鳞癌及正常口腔黏膜组织的差异表达蛋白质,为研究口腔鳞癌发生机制提供实验依据.方法: 收集10 例口腔鳞癌组织及正常口腔黏膜组织,进行二维电泳,选择在表达差异量较大的29 个点进行质谱和生物信息学分析,确定所分析的蛋白质类型. 结果: 口腔鳞癌及相应正常口腔黏膜组织凝胶的平均蛋白质点数分别为2 325±390和2 487±281.双向凝胶电泳图显示,口腔鳞癌及正常口腔黏膜组织的差异表达蛋白质点数为29 个,这29 个点在癌组织中均为低表达,对其进行了质谱(PMF)和生物信息学分析,鉴定了其中的3 个点,它们是:β纤维蛋白(fibrin beta)、磷酸丙糖异构酶(triosephosphate isomerase TIM)、unknown蛋白.结论: β纤维蛋白、磷酸丙糖异构酶、unknown蛋白在口腔鳞癌发生发展过程中发生了改变,其机制尚待进一步阐明.
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