成釉细胞受体介导的内吞功能的研究

摘要

目的:相对定量地研究成釉细胞的内吞功能,并在细胞水平上动态观察成釉细胞的内吞过程,以期为成釉细胞内吞功能研究提供2种新的可行的方法.方法:用视磺酸和地塞米粉(RA/DEX)诱导分泌期成釉细胞LS8细胞成熟,对照组细胞不经诱导处理.分别用激光共聚焦显微镜和活细胞工作站观察细胞内吞荧光标记的FITC-白蛋白的过程.结果:2种方法均显示RA/DEX诱导组细胞内吞活动较对照组强.结论:激光共聚焦显微镜和活细胞工作站都是研究成釉细胞内吞功能的有效方法.%Objective: To study the endocytosis function of ameloblasts relatively and quantitatively and to observe endocytosis process of ameloblasts at celluar level dynamicly.Methods: LS8 ameloblasts were induced by ratinoid acid and dexmethosone(RA/DEX) to be of maturation, and control cells were without treatment.Cells were cultured with FITC-Albumin at 0.1 mg/ml for 30 min.The endocytosis function of the cells was observed by confocal laser scanning microscope and live cell station respectively.Results: Both methods showed that the endocytosis function of RA/DEX group was stronger than that of the control.Conclusion: Both confocal laser scanning microscope and live cell station can be used to study endocytosis function of ameloblasts.